traf6 promoter Search Results


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Fig. 6 <t>TRAF6</t> was directly targeted by miR-1277. A The predicted binding sites between TRAF6 and miR-1277. B, C The interaction between miR- 1277 and TRAF6 was analyzed by dual-luciferase reporter assay and RIP assay. D, E The mRNA and protein levels of TRAF6 in OA cartilage tissues and normal cartilage tissues were determined by qRT-PCR assay and western blot assay, respectively. F The protein level of TRAF6 in different concentrations of IL-1β-treated CHON-001 cells was measured by western blot assay. G The correlation between miR-1277 and TRAF6 in OA cartilage tissues was evaluated by Pearson’s correlation coefficient analysis. H, I The levels of miR-1277 and TRAF6 protein in IL-1β, IL-1β+miR-NC, IL-1β+miR-1277, IL-1β+anti-miR-NC, or IL-1β+anti-miR-1277 treated CHON-001 cells or untreated CHON-001 cells were measured by qRT-PCR assay and western blot assay, respectively. TRAF6 mRNA expression and miR-1277 expression were examined by 2-ΔΔCt method with normalization to GAPDH and U6, respectively. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
Traf6 Traf6, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 6 TRAF6 was directly targeted by miR-1277. A The predicted binding sites between TRAF6 and miR-1277. B, C The interaction between miR- 1277 and TRAF6 was analyzed by dual-luciferase reporter assay and RIP assay. D, E The mRNA and protein levels of TRAF6 in OA cartilage tissues and normal cartilage tissues were determined by qRT-PCR assay and western blot assay, respectively. F The protein level of TRAF6 in different concentrations of IL-1β-treated CHON-001 cells was measured by western blot assay. G The correlation between miR-1277 and TRAF6 in OA cartilage tissues was evaluated by Pearson’s correlation coefficient analysis. H, I The levels of miR-1277 and TRAF6 protein in IL-1β, IL-1β+miR-NC, IL-1β+miR-1277, IL-1β+anti-miR-NC, or IL-1β+anti-miR-1277 treated CHON-001 cells or untreated CHON-001 cells were measured by qRT-PCR assay and western blot assay, respectively. TRAF6 mRNA expression and miR-1277 expression were examined by 2-ΔΔCt method with normalization to GAPDH and U6, respectively. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Journal: Arthritis research & therapy

Article Title: Exosomal circ-BRWD1 contributes to osteoarthritis development through the modulation of miR-1277/TRAF6 axis.

doi: 10.1186/s13075-021-02541-8

Figure Lengend Snippet: Fig. 6 TRAF6 was directly targeted by miR-1277. A The predicted binding sites between TRAF6 and miR-1277. B, C The interaction between miR- 1277 and TRAF6 was analyzed by dual-luciferase reporter assay and RIP assay. D, E The mRNA and protein levels of TRAF6 in OA cartilage tissues and normal cartilage tissues were determined by qRT-PCR assay and western blot assay, respectively. F The protein level of TRAF6 in different concentrations of IL-1β-treated CHON-001 cells was measured by western blot assay. G The correlation between miR-1277 and TRAF6 in OA cartilage tissues was evaluated by Pearson’s correlation coefficient analysis. H, I The levels of miR-1277 and TRAF6 protein in IL-1β, IL-1β+miR-NC, IL-1β+miR-1277, IL-1β+anti-miR-NC, or IL-1β+anti-miR-1277 treated CHON-001 cells or untreated CHON-001 cells were measured by qRT-PCR assay and western blot assay, respectively. TRAF6 mRNA expression and miR-1277 expression were examined by 2-ΔΔCt method with normalization to GAPDH and U6, respectively. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: Circ-BRWD1 short interfering RNA (si-circ-BRWD1) and scramble control (si-NC), the overexpression vector of circ-BRWD1 (circ-BRWD1) and its control (pCD5ciR), miR-1277 mimics (miR-1277) and control mimics (miR-NC), miR-1277 inhibitors (anti-miR-1277) and anti-miR-NC, the overexpression vector of TRAF6 (TRAF6) and pcDNA were purchased from GeneCopoeia.

Techniques: Binding Assay, Luciferase, Reporter Assay, Quantitative RT-PCR, Western Blot, Expressing

Fig. 7 Relationship between miR-1277 and TRAF6 in regulating IL-1β-mediated CHON-001 cell viability, apoptosis, inflammation and ECM degradation. CHON-001 cells were divided into 6 groups: control, IL-1β+miR-NC, IL-1β+miR-1277, IL-1β+miR-1277+pcDNA and IL-1β+miR- 1277+TRAF6. A Western blot assay was utilized for TRAF6 protein level in each group. B–E CCK-8 assay, flow cytometry analysis and EDU assay were conducted for the viability, apoptosis and proliferation of CHON-001 cells. F The protein levels of CyclinD1 and Bax in CHON-001 cells were measured via western blot assay. G ELISA kits were used for the concentrations of IL-6 and IL-8 in CHON-001 cells. H Western blot assay was employed for the protein levels of MMP13 and aggrecan in CHON-001 cells. *P < 0.05,**P < 0.01, ***P < 0.001, ****P < 0.0001

Journal: Arthritis research & therapy

Article Title: Exosomal circ-BRWD1 contributes to osteoarthritis development through the modulation of miR-1277/TRAF6 axis.

doi: 10.1186/s13075-021-02541-8

Figure Lengend Snippet: Fig. 7 Relationship between miR-1277 and TRAF6 in regulating IL-1β-mediated CHON-001 cell viability, apoptosis, inflammation and ECM degradation. CHON-001 cells were divided into 6 groups: control, IL-1β+miR-NC, IL-1β+miR-1277, IL-1β+miR-1277+pcDNA and IL-1β+miR- 1277+TRAF6. A Western blot assay was utilized for TRAF6 protein level in each group. B–E CCK-8 assay, flow cytometry analysis and EDU assay were conducted for the viability, apoptosis and proliferation of CHON-001 cells. F The protein levels of CyclinD1 and Bax in CHON-001 cells were measured via western blot assay. G ELISA kits were used for the concentrations of IL-6 and IL-8 in CHON-001 cells. H Western blot assay was employed for the protein levels of MMP13 and aggrecan in CHON-001 cells. *P < 0.05,**P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: Circ-BRWD1 short interfering RNA (si-circ-BRWD1) and scramble control (si-NC), the overexpression vector of circ-BRWD1 (circ-BRWD1) and its control (pCD5ciR), miR-1277 mimics (miR-1277) and control mimics (miR-NC), miR-1277 inhibitors (anti-miR-1277) and anti-miR-NC, the overexpression vector of TRAF6 (TRAF6) and pcDNA were purchased from GeneCopoeia.

Techniques: Control, Western Blot, CCK-8 Assay, Flow Cytometry, EdU Assay, Enzyme-linked Immunosorbent Assay

Fig. 8 Circ-BRWD1 positively regulated TRAF8 expression by targeting miR-1277 in IL-1β-treated CHON-001 cells. A, B The mRNA and protein levels of TRAF6 in IL-1β, IL-1β+si-NC, IL-1β+si-circ-BRWD1, IL-1β+si-circ-BRWD1+anti-miR-NC, or IL-1β+si-circ-BRWD1+anti-miR-1277 treated or untreated CHON-001 cells were determined by qRT-PCR assay and western blot assay, respectively. TRAF6 mRNA expression was examined by 2-ΔΔCt method with normalization to GAPDH. *P < 0.05, ***P < 0.001, ****P < 0.0001

Journal: Arthritis research & therapy

Article Title: Exosomal circ-BRWD1 contributes to osteoarthritis development through the modulation of miR-1277/TRAF6 axis.

doi: 10.1186/s13075-021-02541-8

Figure Lengend Snippet: Fig. 8 Circ-BRWD1 positively regulated TRAF8 expression by targeting miR-1277 in IL-1β-treated CHON-001 cells. A, B The mRNA and protein levels of TRAF6 in IL-1β, IL-1β+si-NC, IL-1β+si-circ-BRWD1, IL-1β+si-circ-BRWD1+anti-miR-NC, or IL-1β+si-circ-BRWD1+anti-miR-1277 treated or untreated CHON-001 cells were determined by qRT-PCR assay and western blot assay, respectively. TRAF6 mRNA expression was examined by 2-ΔΔCt method with normalization to GAPDH. *P < 0.05, ***P < 0.001, ****P < 0.0001

Article Snippet: Circ-BRWD1 short interfering RNA (si-circ-BRWD1) and scramble control (si-NC), the overexpression vector of circ-BRWD1 (circ-BRWD1) and its control (pCD5ciR), miR-1277 mimics (miR-1277) and control mimics (miR-NC), miR-1277 inhibitors (anti-miR-1277) and anti-miR-NC, the overexpression vector of TRAF6 (TRAF6) and pcDNA were purchased from GeneCopoeia.

Techniques: Expressing, Quantitative RT-PCR, Western Blot